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rnc buffer equilibrated agarose anti flag m2 affinity gel  (Thermo Fisher)


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    Thermo Fisher rnc buffer equilibrated agarose anti flag m2 affinity gel
    Rnc Buffer Equilibrated Agarose Anti Flag M2 Affinity Gel, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agarose+anti+flag+m2+affinity+gel/Agarose/pmc12618258-297-6-13
    Average 99 stars, based on 1 article reviews
    rnc buffer equilibrated agarose anti flag m2 affinity gel - by Bioz Stars, 2026-10
    99/100 stars

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    Article Title: The human ribosome modulates multidomain protein biogenesis by delaying cotranslational domain docking
    Article Snippet: .. Resuspended ribosomal pellets were incubated with RNC buffer-equilibrated agarose ANTI-FLAG® M2 Affinity Gel (ThermoFisher) for 3 h at 4 °C on a spinning wheel (50 μL beads for 50 mL culture). ..



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    ( A ) Plasma TPO level is reduced in LMAN1-deficient compared with WT littermate control mice. ( B ) Tpo mRNA levels were indistinguishable between Lman1 –/– and WT control mice, as demonstrated using 3 different Tpo primer sets. ( C ) <t>FLAG-tagged</t> LMAN1 (LMAN1-FLAG) and myc-tagged TPO (TPO-myc) were expressed in HEK293T cells. A physical interaction between TPO and LMAN1 was suggested, as an <t>anti-FLAG</t> antibody coimmunoprecipitated TPO-myc. In, input (10%); IP, immunoprecipitated fraction. ( D and E ) A reporter human HEK293T cell line that expresses EGFP-fused TPO and mCherry-fused A1AT was generated. ( D ) Deletion of LMAN1 using an LMAN1 -targeting sgRNA resulted in intracellular accumulation of TPO and A1AT compared with cells transduced with a nontargeting (NT) sgRNA. ( E ) Immunofluorescence microscopy demonstrates significantly increased colocalization of TPO in the ER (labeled with blue fluorescent protein) in cells transfected with LMAN1 -targeting sgRNA (LMAN1 KO) compared with control cells (WT). **** P < 0.0001. PCC, Pearson correlation coefficient. ( F ) A reporter cell line expressing TPO fused to mCherry and PCSK9 fused to EGFP was generated. LMAN1 deletion results in intracellular accumulation of TPO but not PCSK9. ( G ) LMAN1 deletion (LMAN1 KO) in HEP3B cells treated with 80 ng/mL IL-6 results in reduced TPO in the supernatant compared with control cells transduced with nontargeting (NT) sgRNA. *** P < 0.001. Statistical analyses in this figure were performed using unpaired Student’s t test.
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    ( A ) Plasma TPO level is reduced in LMAN1-deficient compared with WT littermate control mice. ( B ) Tpo mRNA levels were indistinguishable between Lman1 –/– and WT control mice, as demonstrated using 3 different Tpo primer sets. ( C ) <t>FLAG-tagged</t> LMAN1 (LMAN1-FLAG) and myc-tagged TPO (TPO-myc) were expressed in HEK293T cells. A physical interaction between TPO and LMAN1 was suggested, as an <t>anti-FLAG</t> antibody coimmunoprecipitated TPO-myc. In, input (10%); IP, immunoprecipitated fraction. ( D and E ) A reporter human HEK293T cell line that expresses EGFP-fused TPO and mCherry-fused A1AT was generated. ( D ) Deletion of LMAN1 using an LMAN1 -targeting sgRNA resulted in intracellular accumulation of TPO and A1AT compared with cells transduced with a nontargeting (NT) sgRNA. ( E ) Immunofluorescence microscopy demonstrates significantly increased colocalization of TPO in the ER (labeled with blue fluorescent protein) in cells transfected with LMAN1 -targeting sgRNA (LMAN1 KO) compared with control cells (WT). **** P < 0.0001. PCC, Pearson correlation coefficient. ( F ) A reporter cell line expressing TPO fused to mCherry and PCSK9 fused to EGFP was generated. LMAN1 deletion results in intracellular accumulation of TPO but not PCSK9. ( G ) LMAN1 deletion (LMAN1 KO) in HEP3B cells treated with 80 ng/mL IL-6 results in reduced TPO in the supernatant compared with control cells transduced with nontargeting (NT) sgRNA. *** P < 0.001. Statistical analyses in this figure were performed using unpaired Student’s t test.
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    ( A ) Plasma TPO level is reduced in LMAN1-deficient compared with WT littermate control mice. ( B ) Tpo mRNA levels were indistinguishable between Lman1 –/– and WT control mice, as demonstrated using 3 different Tpo primer sets. ( C ) <t>FLAG-tagged</t> LMAN1 (LMAN1-FLAG) and myc-tagged TPO (TPO-myc) were expressed in HEK293T cells. A physical interaction between TPO and LMAN1 was suggested, as an <t>anti-FLAG</t> antibody coimmunoprecipitated TPO-myc. In, input (10%); IP, immunoprecipitated fraction. ( D and E ) A reporter human HEK293T cell line that expresses EGFP-fused TPO and mCherry-fused A1AT was generated. ( D ) Deletion of LMAN1 using an LMAN1 -targeting sgRNA resulted in intracellular accumulation of TPO and A1AT compared with cells transduced with a nontargeting (NT) sgRNA. ( E ) Immunofluorescence microscopy demonstrates significantly increased colocalization of TPO in the ER (labeled with blue fluorescent protein) in cells transfected with LMAN1 -targeting sgRNA (LMAN1 KO) compared with control cells (WT). **** P < 0.0001. PCC, Pearson correlation coefficient. ( F ) A reporter cell line expressing TPO fused to mCherry and PCSK9 fused to EGFP was generated. LMAN1 deletion results in intracellular accumulation of TPO but not PCSK9. ( G ) LMAN1 deletion (LMAN1 KO) in HEP3B cells treated with 80 ng/mL IL-6 results in reduced TPO in the supernatant compared with control cells transduced with nontargeting (NT) sgRNA. *** P < 0.001. Statistical analyses in this figure were performed using unpaired Student’s t test.
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    ( A ) Plasma TPO level is reduced in LMAN1-deficient compared with WT littermate control mice. ( B ) Tpo mRNA levels were indistinguishable between Lman1 –/– and WT control mice, as demonstrated using 3 different Tpo primer sets. ( C ) <t>FLAG-tagged</t> LMAN1 (LMAN1-FLAG) and myc-tagged TPO (TPO-myc) were expressed in HEK293T cells. A physical interaction between TPO and LMAN1 was suggested, as an <t>anti-FLAG</t> antibody coimmunoprecipitated TPO-myc. In, input (10%); IP, immunoprecipitated fraction. ( D and E ) A reporter human HEK293T cell line that expresses EGFP-fused TPO and mCherry-fused A1AT was generated. ( D ) Deletion of LMAN1 using an LMAN1 -targeting sgRNA resulted in intracellular accumulation of TPO and A1AT compared with cells transduced with a nontargeting (NT) sgRNA. ( E ) Immunofluorescence microscopy demonstrates significantly increased colocalization of TPO in the ER (labeled with blue fluorescent protein) in cells transfected with LMAN1 -targeting sgRNA (LMAN1 KO) compared with control cells (WT). **** P < 0.0001. PCC, Pearson correlation coefficient. ( F ) A reporter cell line expressing TPO fused to mCherry and PCSK9 fused to EGFP was generated. LMAN1 deletion results in intracellular accumulation of TPO but not PCSK9. ( G ) LMAN1 deletion (LMAN1 KO) in HEP3B cells treated with 80 ng/mL IL-6 results in reduced TPO in the supernatant compared with control cells transduced with nontargeting (NT) sgRNA. *** P < 0.001. Statistical analyses in this figure were performed using unpaired Student’s t test.
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    ( A ) Plasma TPO level is reduced in LMAN1-deficient compared with WT littermate control mice. ( B ) Tpo mRNA levels were indistinguishable between Lman1 –/– and WT control mice, as demonstrated using 3 different Tpo primer sets. ( C ) FLAG-tagged LMAN1 (LMAN1-FLAG) and myc-tagged TPO (TPO-myc) were expressed in HEK293T cells. A physical interaction between TPO and LMAN1 was suggested, as an anti-FLAG antibody coimmunoprecipitated TPO-myc. In, input (10%); IP, immunoprecipitated fraction. ( D and E ) A reporter human HEK293T cell line that expresses EGFP-fused TPO and mCherry-fused A1AT was generated. ( D ) Deletion of LMAN1 using an LMAN1 -targeting sgRNA resulted in intracellular accumulation of TPO and A1AT compared with cells transduced with a nontargeting (NT) sgRNA. ( E ) Immunofluorescence microscopy demonstrates significantly increased colocalization of TPO in the ER (labeled with blue fluorescent protein) in cells transfected with LMAN1 -targeting sgRNA (LMAN1 KO) compared with control cells (WT). **** P < 0.0001. PCC, Pearson correlation coefficient. ( F ) A reporter cell line expressing TPO fused to mCherry and PCSK9 fused to EGFP was generated. LMAN1 deletion results in intracellular accumulation of TPO but not PCSK9. ( G ) LMAN1 deletion (LMAN1 KO) in HEP3B cells treated with 80 ng/mL IL-6 results in reduced TPO in the supernatant compared with control cells transduced with nontargeting (NT) sgRNA. *** P < 0.001. Statistical analyses in this figure were performed using unpaired Student’s t test.

    Journal: JCI Insight

    Article Title: LMAN1 serves as a cargo receptor for thrombopoietin

    doi: 10.1172/jci.insight.175704

    Figure Lengend Snippet: ( A ) Plasma TPO level is reduced in LMAN1-deficient compared with WT littermate control mice. ( B ) Tpo mRNA levels were indistinguishable between Lman1 –/– and WT control mice, as demonstrated using 3 different Tpo primer sets. ( C ) FLAG-tagged LMAN1 (LMAN1-FLAG) and myc-tagged TPO (TPO-myc) were expressed in HEK293T cells. A physical interaction between TPO and LMAN1 was suggested, as an anti-FLAG antibody coimmunoprecipitated TPO-myc. In, input (10%); IP, immunoprecipitated fraction. ( D and E ) A reporter human HEK293T cell line that expresses EGFP-fused TPO and mCherry-fused A1AT was generated. ( D ) Deletion of LMAN1 using an LMAN1 -targeting sgRNA resulted in intracellular accumulation of TPO and A1AT compared with cells transduced with a nontargeting (NT) sgRNA. ( E ) Immunofluorescence microscopy demonstrates significantly increased colocalization of TPO in the ER (labeled with blue fluorescent protein) in cells transfected with LMAN1 -targeting sgRNA (LMAN1 KO) compared with control cells (WT). **** P < 0.0001. PCC, Pearson correlation coefficient. ( F ) A reporter cell line expressing TPO fused to mCherry and PCSK9 fused to EGFP was generated. LMAN1 deletion results in intracellular accumulation of TPO but not PCSK9. ( G ) LMAN1 deletion (LMAN1 KO) in HEP3B cells treated with 80 ng/mL IL-6 results in reduced TPO in the supernatant compared with control cells transduced with nontargeting (NT) sgRNA. *** P < 0.001. Statistical analyses in this figure were performed using unpaired Student’s t test.

    Article Snippet: LMAN1 immunoprecipitation was performed using anti-FLAG antibody covalently bonded to agarose beads (EZview Red Anti-FLAG M2 affinity gel, MilliporeSigma).

    Techniques: Clinical Proteomics, Control, Immunoprecipitation, Generated, Transduction, Immunofluorescence, Microscopy, Labeling, Transfection, Expressing